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have observed cross-talk between the calpain and caspase systems during apoptosis, in which calpain activity is indirectly up-regulated by caspase enzymes cleaving calpastatin (Wang et al. 1998; Porn-Ares et al. 1998; Neumar et al. 2003). Calpains have also been shown to impact caspase activity during apoptosis (Nakagawa and Yuan 2000; Chua et al. 2000; Neumar et al. 2003). There is relatively little data, however, on the interaction between caspases and the calpain enzyme system in skeletal muscle as it relates to postmortem proteolysis. A negative relationship between peak caspase 3/7 activity at 8 hours postmortem and calpastatin activity at 0 and 2 days postmortem has been observed in the muscles of normal lambs but not in callipyge lambs (Kemp et al. 2009). Thus, while there is no direct evidence that caspases contribute signi cantly to postmortem tenderization, data suggest that they may play an indirect role by degrading calpastatin. More data is needed, however, to determine de nitively the direct and indirect contribution of the caspase system to postmortem proteolysis and meat tenderization.

Fedewa, MM, Oegema, TR, Jr, Schwartz, MH, MacLeod, A, Lewis, JL (1998) Chondrocytes in culture produce a mechanically functional tissue J Orthop Res 16: 227 236 Fink, C, Ergun, S, Kralisch, D, Remmers, U, Weil, J, Eschenhagen, T (2000) Chronic stretch of engineered heart tissue induces hypertrophy and functional improvement FASEB J 14: 669 679 Frank, EH, and Grodzinsky, AJ (1987) Cartilage electromechanics II A continuum model of cartilage electrokinetics and correlation with experiments J Biomech 20: 629 639 Freed, LE, Marquis, JC, Nohria, A, Emmanual, J, Mikos, AG, Langer, R (1993) Neocartilage formation in vitro and in vivo using cells cultured on synthetic biodegradable polymers J Biomed Mater Res 27: 11 23 Freed, LE, Grande, DA, Lingbin, Z.

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, Emmanual, J, Marquis, JC, Langer, R (1994a) Joint resurfacing using allograft chondrocytes and synthetic biodegradable polymer scaffolds J Biomed Mater Res 28: 891 899 Freed, LE, Marquis, JC, Vunjak-Novakovic, G, Emmanual, J, Langer, R (1994b) Composition of cell-polymer cartilage implants Biotechnol Bioeng 43: 605 614 Freed, LE, Vunjak-Novakovic, G, Biron, R, Eagles, D, Lesnoy, D, Barlow, S, Langer, R (1994c) Biodegradable polymer scaffolds for tissue engineering Bio/Technology 12: 689 693 Freed, LE, and Vunjak-Novakovic, G (1995) Cultivation of cell-polymer constructs in simulated microgravity Biotechnol Bioeng 46: 306 313 Freed, LE, Langer, R, Martin, I, Pellis, N, Vunjak-Novakovic, G (1997) Tissue engineering of cartilage in space Proc Natl Acad Sci USA 94: 13885 13890 Freed, LE, Hollander, AP, Martin, I, Barry, J.

PHYSICS AND VISUAL PERCEPTION The understanding of sensory events involves an understanding of physics. We rely on physics to de ne stimuli such as the electromagnetic radiation that we register as light, the mechanical vibrations that we call sound, the mechanical forces that result in touch, and so forth. The scienti c contributions to our understanding of perception begins with physics, or at least with a protophysics, in which the only measurement instruments available were the eyes, ears, nose, and touch senses of the scientist. Since we learn about the world through the use of our senses, this inevitably leads to a belief that the world is what we perceive it to be an idea that would ultimately come to be abandoned when it became clear that correspondence between percept and reality is not guaranteed. The philosopher-scientists of earlier ages held a presumption consistent with the fact that our faith in the accuracy of our perception seems to be built into the very fabric of our lives as evidenced by homilies such as Seeing is believing. Lucretius (ca. 98 55 B.C.), the Roman philosopher and poet known for his postulation of purely natural causes for earthly phenomena and who tried to prove that fear of the supernatural is consequently without reasonable foundation, stated this article of faith when he asked, What can give us surer knowledge than our senses With what else can we distinguish the true form from the false Thus, we see things as having a color because they are colored. We perceive that a person is larger than a cat because people are larger than cats, and so forth.

R, Langer, R, Vunjak-Novakovic, G (1998) Chondrogenesis in a cell-polymer-bioreactor system Exp Cell Res 240: 58 65 Freed, LE, Martin, I, Vunjak-Novakovic, G (1999) Frontiers in tissue engineering: in vitro modulation of chondrogenesis Clin Orthop Rel Res 367S: S46 S58 Freed, LE, and Vunjak-Novakovic, G (2000a) Tissue engineering bioreactors In Lanza, RP, Langer, R, Vacanti, J, eds, Principles of Tissue Engineering San Diego, Academic Press, pp 143 156 Freed, LE, and Vunjak-Novakovic, G (2000b) Tissue engineering of cartilage In Bronzino, JD, ed, The Biomedical Engineering Handbook Boca Raton, CRC Press, pp 124 126 Gao, J, Niklason, L, Langer, R (1998) Surface hydrolysis of poly(glycolic acid) meshes increases the seeding density of vascular smooth muscle cells J Biomed Mater Res 42: 417 424 Gooch, KJ, Blunk, T, Courter, DL.

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